Thermus aquaticus ATCC 33923 Amylomaltase Gene Cloning and Expression and Enzyme Characterization: Production of Cycloamylose
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چکیده
منابع مشابه
Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization: production of cycloamylose.
The amylomaltase gene of the thermophilic bacterium Thermus aquaticus ATCC 33923 was cloned and sequenced. The open reading frame of this gene consisted of 1,503 nucleotides and encoded a polypeptide that was 500 amino acids long and had a calculated molecular mass of 57,221 Da. The deduced amino acid sequence of the amylomaltase exhibited a high level of homology with the amino acid sequence o...
متن کاملCloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and...
متن کاملcloning and expression of thermus aquaticus dna polymerase gene, using a thermo-inducible expression vector
dna polymerase gene from thermus aquaticus strain yt1 was amplified using venttm dna po-lymerase and cloned under the control of x.pr promoter and expression was induced by a shift in tern perature. the culture was then sonicated, and after centrifugation the lysate was treated with poly ethyleneimine followed by a salting-out step. finally the protein was precipitated with ammonium sulfate and...
متن کاملIsolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus.
The thermostable properties of the DNA polymerase activity from Thermus aquaticus (Taq) have contributed greatly to the yield, specificity, automation, and utility of the polymerase chain reaction method for amplifying DNA. We report the cloning and expression of Taq DNA polymerase in Escherichia coli. From a lambda gt11:Taq library we identified a Taq DNA fragment encoding an epitope of Taq DN...
متن کاملCloning and Expression of Thermus aquaticus DNA polymerase in Escherichia coli
Thermostable DNA polymerase gene from Thermus aquaticus was cloned into constructed Taq from Thermus a Qaticus (pTTQ) plasmid using EcoRI and SalI sites with subsequent transformation in Escherichia coli strain (TOP10). The use of Isopropyl-β-Dthiogalactopyranosid (IPTG) as inducer of interested gene expression under control of the lac promoter was investigated. The optimization of enzyme induc...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.3.910-915.1999